Part:BBa_K4898003

Inducible dual promoter system to produce AlnA under Ptac and rhlAB under Ptet

Background

Alasan and Rhamnolipid are important biosurfactants which are used and can be used in multiple industries, production of these biosurfactants in a certain composition hence becomes essential as producing these biosurfactants in different organisms will require multiple reactors and purification units thus increasing the cost of production, the wild type strains are also pathogenic, for producing these biosurfactants in one organism will allow for these costs to come down hence allowing industries to incorporate these more.

Part Uses

1. This composite part utilises two different inducers to make the biosurfactants in different concentrations governed by the concentration of the inducer put into the broth. 2. The produced biosurfactants are able to chelate metals thus reducing their concentration after treatment. 3. The biosurfactants are also used for ex-situ soil remediation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1353
  Illegal BamHI site found at 1913
  Illegal XhoI site found at 2089
  Illegal XhoI site found at 3375
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2268
  Illegal NgoMIV site found at 2989
  Illegal NgoMIV site found at 3102
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1578
  Illegal BsaI site found at 2618
  Illegal BsaI.rc site found at 1762